Sybr select master mix can be used in either standard or fast cycling mode and is compatible with all applied biosystems real time pcr instruments except the 7900ht real time pcr system. 2x bimaketm sybr green master mix component templatec forward primerd 5 µm reverse primerd 5 µm rox reference dye distilled water dh 2o total reaction volumec amount per reaction µl amount per reaction µl variable 2 5 2 5 1 add to 50 50 variable 1 1 0 4 add to 20 20 1 100 ng 0 2 1 µm 0 2 1 µm 1x protocol component 5 ml 200. When the following instruments are used please choose biotool sybr green master mix high rox with cat.
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